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PEPDIA-INT-065994

Gold

Temporin-SHf

Quality

Source DBs

Activities

Source refs

Literature

Patents

Integrated record

Sequence and molecular profile

FFFLSRIF

Indexed sequence8 aa

1FFFLSRIF

Length

Molecular weight

1076.31 Da

Formula

C57H77N11O10

Structure type

Linear

Synthesis

Ribosomal

Chirality

Family

Gene

Residue composition

Physicochemical profile

Hydrophobic6

Membrane affinity proxy

Polar1

Solubility proxy

Charged1

Net charge proxy +1

Aromatic4

Binding interaction proxy

Versioned scientific descriptors

Physicochemical properties and standard representations

calculated sequence and structure

Reported properties

Legacy compatibility fields are materialized from the versioned PepDiana computed release; no separate source-reported property is available.

Computed properties

physchem-20260620

Molecular weight

1076.2887 Da

Molecular formula

C57H77N11O10

Theoretical pI

9.75

Net charge at pH 7

0.7601

GRAVY

1.775

Instability index

32.825

Aromaticity

0.5

Topological polar surface area

349.15 Å²

cLogP

1.1105

Hydrogen-bond donors

Hydrogen-bond acceptors

Rotatable bonds

Method: PepDiana sequence/structure descriptor pipeline

Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6

Calculated: 2026-06-20T11:01:38.358971+00:00

Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200

Sequence representations

representations-20260620 · RDKit 2024.09.6

ProForma 2.0

validated simple linear

FFFLSRIF

HELM 2.0

validated simple linear

PEPTIDE1{F.F.F.L.S.R.I.F}$$$$V2.0

Canonical SMILES

validated

CCC(C)C(NC(=O)C(CCCNC(=N)N)NC(=O)C(CO)NC(=O)C(CC(C)C)NC(=O)C(Cc1ccccc1)NC(=O)C(Cc1ccccc1)NC(=O)C(N)Cc1ccccc1)C(=O)NC(Cc1ccccc1)C(=O)O

Isomeric SMILES

validated

CC[C@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](N)Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)O

Follow-up research

External analysis tools

Run BLASTPSearch similar protein or peptide sequences at NCBI.Search UniProtLook for related UniProtKB entries using the sequence.Search PubMedFind literature context for this peptide record.Search PubChemOpen chemistry search with the canonical SMILES string.

Links open third-party resources with the current PepDiana sequence, record name, or chemistry notation as the initial query.

Research workflow

Citation and local history

BibTeX

@misc{PEPDIA-INT-065994,
  title = {Temporin-SHf},
  author = {{PepDiana Consortium}},
  year = {2026},
  howpublished = {PepDiana Multifunctional Peptide Database},
  url = {http://127.0.0.1:3004/peptides/PEPDIA-INT-065994},
  note = {Accessed from local PepDiana development build}
}

APA-style note

Temporin-SHf. (2026). PepDiana record PEPDIA-INT-065994. PepDiana Multifunctional Peptide Database. http://127.0.0.1:3004/peptides/PEPDIA-INT-065994

This record is saved to local recent-view history so it can reappear on the peptide browser page.

Integrated source-database annotations

Biological activity (71 records)

Stable record	Activity	Subtype	Value	Target	Assay method	Source
CTPDB_P065994_ACT_apd_0000	Antimicrobial	Anti-Gram+	Not available in the current structured import	Unknown	Not reported by source	AP01534apd / linked
CTPDB_P065994_ACT_apd_0001	Antimicrobial	Antifungal	Not available in the current structured import	Unknown	Not reported by source	AP01534apd / linked
CTPDB_P065994_ACT_apd_0003	Anticancer	-	Not available in the current structured import	Unknown	Not reported by source	AP01534apd / linked
CTPDB_P065994_ACT_camp_0007	Antimicrobial	Antibacterial	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ10688camp / linked
CTPDB_P065994_ACT_camp_0008	Antimicrobial	Anti-Gram+	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ10688camp / linked
CTPDB_P065994_ACT_camp_0009	Antimicrobial	Anti-Gram-	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ10688camp / linked
CTPDB_P065994_ACT_camp_0010	Antimicrobial	Antifungal	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ11922camp / linked
CTPDB_P065994_ACT_camp_0004	Antimicrobial	Antibacterial	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ3773camp / linked
CTPDB_P065994_ACT_camp_0005	Antimicrobial	Anti-Gram+	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ3773camp / linked
CTPDB_P065994_ACT_camp_0006	Antimicrobial	Anti-Gram-	Not available in the current structured import	Unknown	Not reported by source	CAMPSQ3773camp / linked
CTPDB_P065994_ACT_dbaasp_0011	Antimicrobial	-	25 µM	Escherichia coli ATCC 25922	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0012	Antimicrobial	-	>200 µM	Escherichia coli ATCC 35218	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0013	Antimicrobial	-	100 µM	Escherichia coli D21	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0014	Antimicrobial	-	30 µM	Escherichia coli ML-35p	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0015	Antimicrobial	-	>200 µM	Pseudomonas aeruginosa ATCC 27853	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0016	Antimicrobial	-	12.5 µM	Staphylococcus aureus ATCC 25923	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0017	Antimicrobial	-	50 µM	Enterococcus faecalis ATCC 29212	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0018	Antimicrobial	-	3 µM	Bacillus megaterium	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0019	Antimicrobial	-	50 µM	Candida albicans ATCC 90028	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0020	Antimicrobial	-	50 µM	Candida parapsilosis ATCC 22019	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0021	Antimicrobial	-	12.5 µM	Saccharomyces cerevisiae	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0022	Antimicrobial	-	>200 µM	Aspergillus flavus	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0023	Antimicrobial	-	NA	Leishmania infantum (promastigote)	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0024	Antimicrobial	-	NA	Leishmania infantum (amastigote)	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0025	Antimicrobial	-	50 µM	Escherichia coli ATCC 25922	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0026	Antimicrobial	-	>100 µM	Pseudomonas aeruginosa ATCC 27853	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0027	Antimicrobial	-	>100 µM	Acinetobacter baumannii ATCC 19606	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0028	Antimicrobial	-	25 µM	Staphylococcus aureus ATCC 25923	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0029	Antimicrobial	-	25 µM	Staphylococcus aureus ATCC BAA-44	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0030	Antimicrobial	-	25 µM	Streptococcus pyogenes ATCC 19615	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0031	Antimicrobial	-	50 µM	Listeria ivanovii	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0032	Antimicrobial	-	100 µM	Enterococcus faecalis ATCC 29212	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0033	Antimicrobial	-	>200 µM	Human acute monocytic leukemia THP-1	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0034	Antimicrobial	-	50 µM	Staphylococcus aureus ATCC 25923	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0035	Antimicrobial	-	>100 µM	Staphylococcus aureus ATCC 25923	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0036	Antimicrobial	-	>100 µM	Escherichia coli ATCC 25922	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0037	Antimicrobial	-	50 µM	Staphylococcus aureus USA 100	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0038	Antimicrobial	-	50 µM	Staphylococcus aureus USA 300	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0039	Antimicrobial	-	>100 µM	Pseudomonas aeruginosa ATCC 9027	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0040	Antimicrobial	-	>100 µM	Candida albicans ATCC 90028	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0041	Antimicrobial	-	121 µM	Staphylococcus aureus JC114	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0042	Antimicrobial	-	149 µM	Bacillus subtilis JC443	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0043	Antimicrobial	-	116 µM	Escherichia coli JC113	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0044	Antimicrobial	-	88 µM	Pseudomonas aeruginosa JC115	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0045	Antimicrobial	-	>100 µM	Staphylococcus aureus ATCC 43300	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0046	Antimicrobial	-	50 µM	Staphylococcus aureus USA 300	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0047	Antimicrobial	-	100 µM	Staphylococcus aureus	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0048	Antimicrobial	-	>100 µM	Escherichia coli ATCC 25922	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0049	Antimicrobial	-	>100 µM	Escherichia coli	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0050	Antimicrobial	-	>100 µM	Acinetobacter baumannii	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0051	Antimicrobial	-	>100 µM	Klebsiella pneumoniae ATCC 700603	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0052	Antimicrobial	-	>100 µM	Klebsiella pneumoniae	Not reported by source	DBAASPS_3232_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0053	Antimicrobial	-	185 µM	Human acute monocytic leukemia THP-1	Not reported by source	DBAASPS_9485_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0054	Antimicrobial	-	25 µM	Staphylococcus aureus ATCC 25923	Not reported by source	DBAASPS_9485_chain0dbaasp / linked
CTPDB_P065994_ACT_dbaasp_0055	Antimicrobial	-	50 µM	Escherichia coli ATCC 25922	Not reported by source	DBAASPS_9485_chain0dbaasp / linked
CTPDB_P065994_ACT_dramp_0056	Antimicrobial	-	Not available in the current structured import	Unknown	Not reported by source	DRAMP20778dramp / linked
CTPDB_P065994_ACT_dramp_0057	Antimicrobial	Antibacterial	Not available in the current structured import	Unknown	Not reported by source	DRAMP20778dramp / linked
CTPDB_P065994_ACT_dramp_0058	Antimicrobial	Anti-Gram+	Not available in the current structured import	Unknown	Not reported by source	DRAMP20778dramp / linked
CTPDB_P065994_ACT_dramp_0059	Antimicrobial	Anti-Gram-	Not available in the current structured import	Unknown	Not reported by source	DRAMP20778dramp / linked
CTPDB_P065994_ACT_dramp_0060	Antimicrobial	Antifungal	Not available in the current structured import	Unknown	Not reported by source	DRAMP20778dramp / linked
CTPDB_P065994_ACT_dramp_0063	Antimicrobial	-	Not available in the current structured import	Unknown	Not reported by source	DRAMP27646dramp / linked
CTPDB_P065994_ACT_dramp_0061	Antimicrobial	-	Not available in the current structured import	Unknown	Not reported by source	DRAMP34402dramp / linked
CTPDB_P065994_ACT_dramp_0062	Anticancer	-	Not available in the current structured import	Unknown	Not reported by source	DRAMP34402dramp / linked
CTPDB_P065994_ACT_hemolytik_0064	Toxicity	Hemolytic	200.0 µM	Erythrocytes	Not reported by source	1501hemolytik / linked
CTPDB_P065994_ACT_satpdb_0065	Antimicrobial	Antibacterial	Not available in the current structured import	Unknown	Not reported by source	satpdb18512satpdb / linked
CTPDB_P065994_ACT_satpdb_0066	Antimicrobial	-	Not available in the current structured import	Unknown	Not reported by source	satpdb18512satpdb / linked
CTPDB_P065994_ACT_satpdb_0067	Antimicrobial	Antifungal	Not available in the current structured import	Unknown	Not reported by source	satpdb18512satpdb / linked
CTPDB_P065994_ACT_satpdb_0068	Antimicrobial	Antibacterial	Not available in the current structured import	Unknown	Not reported by source	satpdb23862satpdb / linked
CTPDB_P065994_ACT_satpdb_0069	Toxicity	General Toxic	Not available in the current structured import	Unknown	Not reported by source	satpdb23862satpdb / linked
CTPDB_P065994_ACT_satpdb_0070	Antimicrobial	-	Not available in the current structured import	Unknown	Not reported by source	satpdb23862satpdb / linked
CTPDB_P065994_ACT_satpdb_0071	Antimicrobial	Antifungal	Not available in the current structured import	Unknown	Not reported by source	satpdb23862satpdb / linked

Sequence-linked cross-layer evidence

Linked primary-literature evidence

Records

Papers

Positive

Negative

Primary identities

These observations were collected directly from primary literature and linked by identical normalized sequence. They remain a separate evidence layer and do not overwrite Integrated source-database annotations.

View PEPDIA-PLP-003483 View all experimental records

Provenance

Source database records (12)

Source-specific sequence or modification context. One or more source records use a sequence notation that differs from the canonical backbone. Open each raw sequence before interpreting stereochemistry, terminal groups, or non-standard residues.

apd

AP01534

Raw sequence

FFFLSRIF

camp

CAMPSQ3773

Raw sequence

FFFLSRIF

camp

CAMPSQ10688

Raw sequence

FFFLSRIF

camp

CAMPSQ11922

Raw sequence

FFFLSRIF

dbaasp

DBAASPS_3232_chain0

Raw sequence

FFFLSRIF

dbaasp

DBAASPS_9485_chain0

Raw sequence

ffflsrif

dramp

DRAMP20778

Raw sequence

FFFLSRIF

dramp

DRAMP34402

Raw sequence

ffflsrif

dramp

DRAMP27646

Raw sequence

FFFLSRIF

hemolytik

1501

Raw sequence

FFFLSRIF

satpdb

satpdb18512

Raw sequence

FFFLSRIF

satpdb

satpdb23862

Raw sequence

FFFLSRIF

Citation trail

Literature (5 records)

Temporin-SHf, a new type of phe-rich and hydrophobic ultrashort antimicrobial peptide

Abbassi F, Lequin O, Piesse C, Goasdoue N, Foulon T, Nicolas P, Ladram A.2010

J Biol Chem2010PMID 20308076

-

--PMID 28105725

Temporin-SHf, a new type of phe-rich and hydrophobic ultrashort antimicrobial peptide.

Abbassi F, Lequin O, Piesse C, Goasdoué N, Foulon T, Nicolas P, Ladram A

J Biol Chem2010PMID 26181487

Temporin-SHf, a new type of phe-rich and hydrophobic ultrashort antimicrobial peptide.

Abbassi F, Lequin O, Piesse C, Goasdoué N, Foulon T, Nicolas P, Ladram A

J Biol Chem2010PMID 30924306

Temporin-SHf, a new type of phe-rich and hydrophobic ultrashort antimicrobial peptide.

Abbassi F, Lequin O, Piesse C, Goasdoué N, Foulon T, Nicolas P, Ladram A

J Biol Chem2010PMID 37980400

IP evidence

Patents (1)

US 20190048052

dramp

Audit surface

Raw annotations and release metadata

Integrated record last updated: 2026-04-15T00:59:11.491115+00:00

Modification summary

No structured modification annotations are available for this record.

ADMET

{
  "toxicity": [
    {
      "unit": "µM",
      "value": "200",
      "source_db": "dbaasp",
      "assay_method": "50% Hemolysis",
      "toxicity_type": "Hemolytic",
      "target_cellline": "Human erythrocytes"
    },
    {
      "unit": "µM",
      "value": "157",
      "source_db": "dbaasp",
      "assay_method": "IC50",
      "toxicity_type": "Cytotoxic",
      "target_cellline": "Human embryonic kidney HEK293 cells"
    },
    {
      "unit": "µM",
      "value": ">200",
      "source_db": "dbaasp",
      "assay_method": "50% Hemolysis",
      "toxicity_type": "Hemolytic",
      "target_cellline": "Rat erythrocytes"
    },
    {
      "unit": "µM",
      "value": ">100",
      "source_db": "dbaasp",
      "assay_method": "10% Hemolysis",
      "toxicity_type": "Hemolytic",
      "target_cellline": "Sheep erythrocytes"
    },
    {
      "unit": "µM",
      "value": "59",
      "source_db": "dbaasp",
      "assay_method": "IC50",
      "toxicity_type": "Cytotoxic",
      "target_cellline": "Human embryonic kidney HEK293T cells"
    },
    {
      "unit": "µM",
      "value": "164",
      "source_db": "dbaasp",
      "assay_method": "IC50",
      "toxicity_type": "Cytotoxic",
      "target_cellline": "Human embryonic kidney HEK293 cells"
    },
    {
      "unit": "µM",
      "source_db": "dramp",
      "assay_method": "[Ref.20308076] Temporin-SHf is not cytotoxic against human erythrocytes (LC50 = 200 μm), with a hemolytic activity detected only at peptide concentration far above the MIC values determined for the sensitive strains (3–50 μm)",
      "toxicity_type": "Hemolytic",
      "target_cellline": "Erythrocytes"
    },
    {
      "unit": "µM",
      "source_db": "dramp",
      "assay_method": "[Ref.20308076] No cytotoxicity information found",
      "toxicity_type": "Cytotoxic"
    }
  ]
}

Source metadata

[
  {
    "source_db": "apd",
    "organism_name": "Unknown"
  },
  {
    "source_db": "camp",
    "organism_name": "Unknown"
  },
  {
    "source_db": "dbaasp",
    "organism_name": "Unknown"
  },
  {
    "source_db": "dramp",
    "organism_name": "Unknown"
  },
  {
    "source_db": "hemolytik",
    "organism_name": "Unknown"
  },
  {
    "source_db": "satpdb",
    "organism_name": "Unknown"
  }
]

Structure metadata

{
  "source_db": "satpdb",
  "dssp_states": "CCGGGGGC"
}