Poultry Science · 2026
Sequence evidence
KFLCSIPGMYYFAYHLTV
A peptide derived from the adiponectin amino acid sequence of geese, named gADP3 (KFLCSIPGMYYFAYHLTV), was designed... The designed peptide gADP3 was synthesized using the PepPower™ chemical peptide synthesis technique by Nanjing GenScript Biotechnology Corporation.
Experimental observations
gADP3 and AdipoRon interventions led to a significant reduction in IL-6 mRNA expression in comparison with the LPS group (p < 0.01) (Fig. 4A).
gADP3 and AdipoRon interventions led to a substantial reduction in TNF-α expression (p < 0.01) (Fig. 4B).
gADP3 intervention increased IL-10 mRNA levels in the thymus (p < 0.05) (Fig. 4C).
both gADP3 and AdipoRon interventions led to a significant reduction in the levels of IL-6 and TNF-α in spleen tissue (p < 0.01) (Fig. 4D,E).
gADP3 intervention significantly increased IL-10 levels (p < 0.01) (Fig. 4F).
gADP3 and AdipoRon interventions led to a significant reduction in MDA content (p < 0.05) (Fig. 5A).
gADP3 and AdipoRon interventions led to a substantial enhancement in GSH-Px activity (p < 0.05) (Fig. 5B).
intervention with gADP3 and AdipoRon led to a substantial reduction in MDA levels (p < 0.01) (Fig. 5D).
following gADP3 intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the thymus were significantly reduced (p < 0.05) (Fig. 8A,B,C).
following gADP3 intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the thymus were significantly reduced (p < 0.05) (Fig. 8A,B,C).
following gADP3 intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the thymus were significantly reduced (p < 0.05) (Fig. 8A,B,C).
gADP3 and AdipoRon interventions significantly reduced NF-κB p65 and MyD88 protein expression levels (p < 0.05) (Fig. 8D,E,F,G).
gADP3 and AdipoRon interventions significantly reduced NF-κB p65 and MyD88 protein expression levels (p < 0.05) (Fig. 8D,E,F,G).
following gADP3 and AdipoRon intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the spleen were significantly reduced (p < 0.05) (Fig. 9A,B,C).
following gADP3 and AdipoRon intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the spleen were significantly reduced (p < 0.05) (Fig. 9A,B,C).
following gADP3 and AdipoRon intervention, mRNA expression levels of NF-κB p65, MyD88, and TLR4 in the spleen were significantly reduced (p < 0.05) (Fig. 9A,B,C).
gADP3 and AdipoRon interventions significantly reduced the expression levels of these two proteins (p < 0.05) (Fig. 9D,E,F,G).
gADP3 and AdipoRon interventions significantly reduced the expression levels of these two proteins (p < 0.05) (Fig. 9D,E,F,G).
Subsequent to the administration of gADP3 and AdipoRon, a marked increase in AdipoR1 mRNA expression levels was observed in the thymus (p < 0.05) (Fig. 6A).
gADP3 intervention resulted in a significant increase in AdipoR2 mRNA expression levels in the thymus (p < 0.05) (Fig. 6B).
the intervention with gADP3 also elevated AdipoR2 mRNA expression in the spleen (p < 0.01) (Fig. 6G).
gADP3 intervention significantly increased AMPK mRNA levels (p < 0.05) (Fig. 7A).
gADP3 intervention significantly increased AMPK mRNA and protein levels in the spleen (p < 0.01) (Fig. 7D).
after interventions with AdipoRon and gADP3, both IgA and IgG levels were significantly elevated (p < 0.05) (Fig. 1C,D).
gADP3 intervention significantly elevated T4 and TSH levels (p < 0.05) (Fig. 1E,F).
after the injection of AdipoRon and gADP3, both increased the levels of ALB and TP in the blood (p < 0.01) (Fig. 1A,B).
gADP3 intervention... showed no statistically significant effect on COR (p > 0.05) (Fig. 1G).
intervention with gADP3 and AdipoRon did not mitigate the decrease in GSH-Px activity in spleen tissue induced by LPS (Fig. 5E).
Western blot analysis of thymus tissue revealed no significant changes in protein levels of either AdipoR1 or AdipoR2 (p > 0.05) (Fig. 6C,D,E).
Western blot analysis of thymus tissue revealed no significant changes in protein levels of either AdipoR1 or AdipoR2 (p > 0.05) (Fig. 6C,D,E).
gADP3 intervention significantly increased AMPK mRNA levels (p < 0.05) and also elevated AMPK expression levels, although without significant difference (p > 0.05) (Fig. 7A,B,C).
changes in TLR4 protein levels in the thymus were not significant (p > 0.05) (Fig. 8D,E,F,G).
changes in TLR4 protein levels in spleen tissue were not significant (p > 0.05) (Fig. 9D,E,F,G).
Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.
