P1

Table 1: Hydrostatin-AMP2 MIC against Escherichia coli is 16 μg/mL.

Table 1: Hydrostatin-AMP2 MBC against Escherichia coli is 32 μg/mL (reported as MIC/MBC 16/32).

Table 1: Hydrostatin-AMP2 MIC against Klebsiella pneumoniae 44 is 32 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against K. pneumoniae 48 is 16 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against K. pneumoniae 49 is 32 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against K. pneumoniae 50 is 32 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against K. pneumoniae 51 is 32 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against Propionibacterium acnes is 32 μg/mL.

Table 1: Hydrostatin-AMP2 MIC against Staphylococcus aureus is 32 μg/mL.

Table 2: Control MIC of Hydrostatin-AMP2 against E. coli is 16 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 50 mM NaCl is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 100 mM NaCl is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 150 mM NaCl is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 200 mM NaCl is 64 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 1 mM CaCl2 is 64 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 2 mM CaCl2 is 64 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli in 4 mM CaCl2 is 128 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 1h in human serum is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 2h in human serum is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 3h in human serum is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 4h in human serum is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 4°C treatment is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 37°C treatment is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 80°C treatment is 32 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli after 100°C treatment is 64 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli at pH 2 is 64 μg/mL.

Table 2: MIC of Hydrostatin-AMP2 against E. coli at pH 12 is 64 μg/mL.

Table 3: Cell death of L929 cells treated with Hydrostatin-AMP2 is 8.08%.

Table 3: Cell death of HaCaT cells treated with Hydrostatin-AMP2 is 5.89%.

Table 3: Cell death of RAW264.7 cells treated with Hydrostatin-AMP2 is 10.81%.

Table 3: Hemolysis of human erythrocytes by Hydrostatin-AMP2 is 4.6%.

Section 2.6: The inhibition of Hydrostatin-AMP2 on the biofilm formation of S. aureus reached 71.02% at the concentration of 2 × MIC.

Section 2.6: When the peptide concentration changed from 4 × MIC to 8 × MIC, the scavenging effect of Hydrostatin-AMP2 on mature biofilms increased from 50.02% to 60.99%.

Section 2.6: When the peptide concentration changed from 4 × MIC to 8 × MIC, the scavenging effect of Hydrostatin-AMP2 on mature biofilms increased from 50.02% to 60.99%.

Section 2.10: 20 μg/mL of Hydrostatin-AMP2 could reduce iNOS expression by 87.5%.