Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 548
Aptamer sequence: TACCGGGTAATATGCACACATGCTGATAGCAAGCAATGCA
Target unique ID: 5327
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_946 SMZ5 Molecule Sulfamethazine 185.7 nM 20 mM Tris-HCl, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.4 33184760
Structure information of aptamer
Aptamer Sequence: TACCGGGTAATATGCACACATGCTGATAGCAAGCAATGCA
The optimal secondary structure in dot-bracket notation: ............((((....((((.......)))).))))
The centroid secondary structure in dot-bracket notation: ............((((....((((.......)))).))))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_946 Description SMZ5
Aptamer chemistry DNA Length 40 nt
GC content 45.0% Molecular weight 12,321.97 Da
Molarity of 1 μg/μl solution 81.16 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target recognition
Sequence TACCGGGTAATATGCACACATGCTGATAGCAAGCAATGCA
Applications Aptasensor can be used to sensitively, selectively, and accurately detect SMZ residues in foods
Target information
Type Detail Type Detail
PubChem ID 5327 Molecular name Sulfamethazine
Molecular formula C12H14N4O2S Molecular weight 278.33
IUPAC name 4-amino-N-(4,6-dimethylpyrimidin-2-yl)benzenesulfonamide InChIKey ASWVTGNCAZCNNR-UHFFFAOYSA-N
LogP 1.476 Topological polar surface area 97.97
Hydrogen bond acceptor 5 Hydrogen bond donor 2

Synonym(s):
sulfamethazine; Sulfadimidine; 57-68-1; Sulphamethazine; Sulfadimethyldiazine; Sulfamezathine; Sulfadimerazine; 4-amino-N-(4,6-dimethylpyrimidin-2-yl)benzenesulfonamide; Sulphamezathine; Sulfadimezine; Sulfadimidin; Sulphadimidine; Sulfadimethylpyrimidine; Sulphadimethylpyrimidine; Sulfadimesin; Sulfadimesine; Sulfadimezin; Sulfametazyny; Sulfamethiazine; Sulphamethasine; Sulphamidine; Sulphodimezine; Sulfadine; Cremomethazine; Sulfadimidina; Sulfadimidinum; Sulfametazina; Sulfodimezine; Azolmetazin; Dimezathine; Intradine; Kelametazine; Pirmazin; Spanbolet; Sulfodimesin; Superseptil; Superseptyl; Vertolan; Diazil; Mermeth; Neasina; Neazina; Sulfa-Isodimerazine; Dimidin-R; Hava-Span; Calfspan Tablets; Sa III; 4,6-Dimethyl-2-sulfanilamidopyrimidine; 4-Amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide; 2-Sulfanilamido-4,6-dimethylpyrimidine; SulfaSURE SR Bolus; N-(4,6-Dimethyl-2-pyrimidyl)sulfanilamide; 4,6-Dimethylsulfadiazine; Diazil (the sulfanilamide); Primazin; 2-(p-Aminobenzenesulfonamido)-4,6-dimethylpyrimidine; Benzenesulfonamide, 4-amino-N-(4,6-dimethyl-2-pyrimidinyl)-; 4-Amino-N-(2,6-dimethyl-4-pyrimidinyl)benzenesulfonamide; A-502; 6-(4'-Aminobenzol-sulfonamido)-2,4-dimethylpyrimidin; N(1)-(4,6-Dimethyl-2-pyrimidyl)sulfanilamide; NCI-C56600; N(1)-(4,6-Dimethyl-2-pyrimidinyl)sulfanilamide; Sulmet; Sulfadimidine;Sulfadimerazine; (p-Aminobenzolsulfonyl)-2-amino-4,6-dimethylpyrimidin; Sulfamethazine (USP); Sulfamethazine [USP]; UNII-48U51W007F; 2-(4-Aminobenzenesulfonamido)-4,6-dimethylpyrimidine; CHEBI:102265; Sulfadimezinum; MFCD00006066; Sulfanilamide, N(sup1)-(4,6-dimethyl-2-pyrimidinyl)-; MLS000069711; 4-Amino-N-(4,6-dimethyl-pyrimidin-2-yl)-benzenesulfonamide; Solfadimidina; n(sup1)-(2,6-Dimethylpyrimid-4-yl)sulfanilamide; N(Sup1)-(4,6-Dimethyl-2-pyrimidyl)sulfanilamide; NSC67457; Sulfadimidine-d4; Sulfanilamide, N1-(4,6-dimethyl-2-pyrimidinyl)-; Sulka S Boluses; N(Sup1)-(4,6-Dimethyl-2-pyrimidinyl)sulfanilamide; NSC683529; 48U51W007F; NCGC00018243-07; SMR000017409; Solfadimidina [DCIT]; Sulfametazyny [Polish]; DSSTox_CID_1290; DSSTox_RID_76062; DSSTox_GSID_21290; SMZ; Sulfadimidinum [INN-Latin]; Sulfadimidina [INN-Spanish]; Sulfamidine; Sulfametazina [Italian]; Sulfadimidine [INN:BAN]; CAS-57-68-1; BN 2409; CCRIS 3701; Sulfamezathine (TN); Sulfadimidine (INN); HSDB 4157; EINECS 200-346-4; NSC 67457; BRN 0261304; N(sup 1)-(4,6-Dimethyl-2-pyrimidyl)sulfanilamide; sulfamethazone; Diazilsulfadine; N(sup 1)-(4,6-Dimethyl-2-pyrimidinyl)sulfanilamide; Calfspan; Panazin; AI3-26817; Sulka k boluses; S-Dimidine; Dimidim-R; 4-amino-N-(4,6-dimethylpyrimidin-2-yl)benzene-1-sulfonamide; (p-Aminobenzolsulfonyl)-2-amino-4,6-dimethylpyrimidin [German]; 6-(4'-Aminobenzol-sulfonamido)-2,4-dimethylpyrimidin [German]; Sulfadimidine,(S); Sulfanilamide, N(1)-(4,6-dimethyl-2-pyrimidinyl)-; Sulfadimidine-13C6; 4-Amino-N-(4,6-dimethyl-2-pyrimidyl)benzenesulfonamide; Sulfanilamide, N(sup1)-(2,6-dimethyl-4-pyrimidinyl)-; Sentry aq mardel biospheres maracyn plus; Spectrum_000990; [(4-Aminophenyl)sulfonyl](4,6-dimethylpyrimidin-2-yl)amine; 4-amino-N-(4; Opera_ID_1374; Prestwick0_000775; Prestwick1_000775; Prestwick2_000775; Prestwick3_000775; Spectrum2_001321; Spectrum3_001700; Spectrum4_000344; Spectrum5_001270; Sulfamethazine, >=99%; CHEMBL446; Epitope ID:122238; Cambridge id 5251384; NCIOpen2_003489; BIDD:PXR0093; Oprea1_142608; Oprea1_677935; BSPBio_000850; BSPBio_003260; CBDivE_012932; KBioGR_000747; KBioSS_001470; 5-25-10-00250 (Beilstein Handbook Reference); MLS000103403; MLS001077331; MLS002454449; DivK1c_000293; SCHEMBL151305; SPECTRUM1500548; SPBio_001441; SPBio_002789; BPBio1_000936; DTXSID6021290; Sulfanilamide, N(sup 1)-(4,6-dimethyl-2-pyrimidinyl)-; ASWVTGNCAZCNNR-UHFFFAOYSA-; HMS500O15; KBio1_000293; KBio2_001470; KBio2_004038; KBio2_006606; KBio3_002480; ZINC57494; NINDS_000293; HMS1921A17; HMS2092I19; HMS3652K03; Pharmakon1600-01500548; ALBB-033473; BCP28439; HY-B0035; Tox21_110847; Tox21_202221; Tox21_303006; BBL005404; CCG-39259; NSC-67457; NSC757326; s3133; STK097514; AKOS000119894; Tox21_110847_1; DB01582; MCULE-7831442410; MS-1576; NSC-683529; NSC-757326; IDI1_000293; NCGC00018243-01; NCGC00018243-02; NCGC00018243-03; NCGC00018243-04; NCGC00018243-05; NCGC00018243-06; NCGC00018243-08; NCGC00018243-09; NCGC00021490-03; NCGC00021490-04; NCGC00021490-05; NCGC00021490-06; NCGC00256371-01; NCGC00259770-01; Sulfadimidine solution, 1 mg/mL in H2O; WLN: T6N CNJ BMSWR DZ& D1 F1; AC-16126; H462; Sulfanilamide,6-dimethyl-4-pyrimidinyl)-; SBI-0051522.P003; SULFAMETHAZINE (TRISULFAPYRIMIDINES); FT-0655603; FT-0674743; N1-(4,6-Dimethyl-2-pyrimidyl)sulfanilamide; SW219689-1; Benzenesulfonamide,6-dimethyl-4-pyrimidinyl)-; Sulfamethazine 100 microg/mL in Acetonitrile; C19530; D02436; N1-(4,6-Dimethyl-2-pyrimidinyl)sulfanilamide; AB00052097_12; AB00052097_13; A831551; SR-01000000211; Sulfamethazine, Vetec(TM) reagent grade, >=99%; Sulfamethazine, VETRANAL(TM), analytical standard; Q3976823; SR-01000000211-3; W-105450; 4-amino-N-(4,6-dimethyl-2-pyridyl)benzenesulfonamide; BRD-K11640013-001-02-6; BRD-K11640013-236-03-6; 2-(4-Aminobenzenesulfonylamino)-4,6-dimethylpyrimidine; F1443-4796; N(1)-(4,6-Dimethyl-2-pyrimidinyl)sulfanilamide sulfadimidine; Sulfadimidine, European Pharmacopoeia (EP) Reference Standard; (4-AMINO-N-(4,6-DIMETHYL-2-PYRIMIDINYL)BENZENE SULFONAMIDE; 4-amino-N~1~-(4,6-dimethyl-2-pyrimidinyl)-1-benzenesulfonamide; HSDB 4157; HSDB 4157; HSDB 4157;Sulfadimidine;Sulfadimerazine; Sulfamethazine, United States Pharmacopeia (USP) Reference Standard; Sulfadimidine for peak identification, European Pharmacopoeia (EP) Reference Standard

Activity data
Interaction ID 548
Target type Molecule
Target unique ID 5327
Activity 185.7 nM
Binding Conditions/Buffer

20 mM Tris-HCl, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.4
Assay

The relative fluorescence intensity versus concentration of the FAM-labeled aptamers. The dissociation constants of aptamers were used to determine by nonlinear regression analysis. The relative fluorescence intensity is expressed as (F-F0), where F and F0 are the fluorescence intensity obtained with the SMZ analyte and the blank sample, respectively
PubMed ID 33184760
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_335 DNA ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG 56.00%
Apta_302 DNA ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT 55.00%
Apta_884 DNA ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT 54.10%
Apta_395 DNA AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA 54.00%
Apta_573 DNA CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA 53.33%
Apta_883 DNA CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA 53.33%
Apta_309 DNA TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC 53.06%
Apta_309 DNA TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC 53.06%
Apta_1162 DNA AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT 53.06%
Apta_322 DNA GAAGTGAAAATGACAGAACACAACA 52.50%
Apta_323 DNA GGCTATAGCACATGGGTAAAACGAC 52.50%
Apta_325 DNA TACTGCATGCACACCACTTCAACTA 52.50%
Apta_323 DNA GGCTATAGCACATGGGTAAAACGAC 52.50%
Apta_322 DNA GAAGTGAAAATGACAGAACACAACA 52.50%
Apta_323 DNA GGCTATAGCACATGGGTAAAACGAC 52.50%
Apta_325 DNA TACTGCATGCACACCACTTCAACTA 52.50%
Apta_850 DNA CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA 52.50%
Apta_873 DNA CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG 52.50%
Apta_965 DNA CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA 52.50%
Apta_340 DNA TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA 52.27%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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