Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 326
Aptamer sequence: GGTTGGTGTGGTTGGTTTTTTTTTTTTTTTTTTTTAGTCCGTGGTAGGGCAGGTTGGGGTGACT
Target unique ID: P15692
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_317 | 15mer-29-mer dimer with 20T linker | Protein | VEGFA_HUMAN | 0.35 nM | 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0 | 27879754 |
Structure information of aptamer
| Aptamer Sequence: | GGTTGGTGTGGTTGGTTTTTTTTTTTTTTTTTTTTAGTCCGTGGTAGGGCAGGTTGGGGTGACT |
| The optimal secondary structure in dot-bracket notation: | ....................................((((......)))).............. |
| The centroid secondary structure in dot-bracket notation: | ....................................((((......)))).............. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_317 | Description | 15mer-29-mer dimer with 20T linker |
| Aptamer chemistry | DNA | Length | 64 nt |
| GC content | 42.2% | Molecular weight | 20,197.64 Da |
| Molarity of 1 μg/μl solution | 49.51 μM | Number of G-quadruplexes | 2 |
| G-Score | 20 | Function | Targeted binding |
| Sequence | GGTTGGTGTGGTTGGTTTTTTTTTTTTTTTTTTTTAGTCCGTGGTAGGGCAGGTTGGGGTGACT | ||
| Applications | Improves the affinities | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P15692 | Protein name | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
| Gene name(s) | VEGFA VEGF | Organism | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
| Status | reviewed in uniprot database | Length | 232 |
| Mass | 27,042 | Activity regulation | N/A |
| Involvement in disease | Microvascular complications of diabetes 1 (MVCD1) | Catalytic activity | N/A |
| PDB ID(s) | 1BJ1;1CZ8;1FLT;1KAT;1KMX;1MJV;1MKG;1MKK;1QTY;1TZH;1TZI;1VGH;1VPF;1VPP;2FJG;2FJH;2QR0;2VGH;2VPF;3BDY;3P9W;3QTK;3S1B;3S1K;3V2A;4DEQ;4GLN;4GLS;4KZN;4QAF;4WPB;4ZFF;5DN2;5FV1;5FV2;5HHC;5HHD;5O4E;5T89;6BFT;6D3O;6T9D;6V7K;6Z13;6Z3F;6ZBR;6ZCD;6ZFL;7KEZ;7KF0;7KF1;7LL8;7LL9; |
 |
Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 326 |
| Target type | Protein | |
| Target unique ID | P15692 | |
| Activity | 0.35 nM | |
| Binding Conditions/Buffer | 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0 |
|
| Assay | N/A |
|
| PubMed ID | 27879754 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_785 | DNA | ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT | |
| Apta_596 | DNA | GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG | |
| Apta_786 | DNA | ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT | |
| Apta_787 | DNA | ATCCAGAGTGACGCAGCATTTGTTTAATATGTTTTTTAATTCCCCTTGTGGTGTGTTGTGGACACGGTGGCTTAGT | |
| Apta_1174 | DNA | ATGAGAGCGTCGGTGTGGTAAGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACTCTTGCTGTAGGAGGGTGCGGAAGTA | |
| Apta_493 | DNA | GGGGTGGTGGGTTCTTTTTGTGGTATTGGTGTGGGGGGCA | |
| Apta_1109 | DNA | GGTATGTGTCGTTGATCTGGATATCTATGCCTAATGCTCGTAGGCGACTGTTCGGATAGTAACGTTGACCGCATAC | |
| Apta_127 | DNA | GGTATTGAGGGTCGCATCCACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGTGATGGCTCTAACTCTCCTCT | |
| Apta_784 | DNA | ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT | |
| Apta_1154 | DNA | TTAATTTTTTGATCTTTGATTGTTTTATTTTTTTTATTGTTTGTG | |
| Apta_1283 | DNA | TACTGATGCAGGGTAATGCTTCTGTGTTTGTTCTTCATCATTAACGGCCGCGTCTGGCGG | |
| Apta_732 | DNA | AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT | |
| Apta_1175 | DNA | ATGAGAGCGTCGGTGTGGTATCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGAGGGTGCGGAAGTA | |
| Apta_555 | DNA | TGTACCGTCTGAGCGATTCGTACGGATTGGGTTTGCACTTTACCTGCGGTGCATCGCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_841 | DNA | TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC | |
| Apta_842 | DNA | TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC | |
| Apta_1235 | DNA | TGTACCGTCTGAGCGATTCGTACGATCGGGTCGGGGTCTATTTTAGGGCCATTTGGCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_128 | DNA | GGTATTGAGGGTCGCATCCCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCGGATGGCTCTAACTCTCCTCT | |
| Apta_1088 | DNA | ATCCAGAGTGACGCAGCATCGAGTGGCTTGTTTGTAATGTAGGGTTCCGGTCGTGGGTTGGACACGGTGGCTTAGT | |
| Apta_1089 | DNA | ATCCAGAGTGACGCAGCAATTACCGATGGCTTGTTTGTAATGTAGGGTTCCGTCGGATTGGACACGGTGGCTTAGT |
DEV TOOL
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