ProForma 2.0
validated simple linear
LHKFR
Back to Primary literature
Primary-literature peptidehigh evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001765
LR5
Aliases: LR5
Normalized sequence
LHKFR
5 amino acids
Release PepDiana-V1-20260620
6
Activity records
1
Source papers
2
Positive
4
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
699.844 Da
Molecular formula
C33H53N11O6
Theoretical pI
11.0008
Net charge at pH 7
1.8463
GRAVY
-1
Instability index
55.36
Aromaticity
0.2
Topological polar surface area
296.32 Ų
cLogP
-1.0156
Hydrogen-bond donors
11
Hydrogen-bond acceptors
9
Rotatable bonds
23
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{L.H.K.F.R}$$$$V2.0
Canonical SMILES
validated
CC(C)CC(N)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CCCCN)C(=O)NC(Cc1ccccc1)C(=O)NC(CCCNC(=N)N)C(=O)O
Isomeric SMILES
validated
CC(C)C[C@H](N)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O
Paper-level observations
Experimental evidence grouped by activity
Immunomodulatory 4 records
PMC10221837
4 observationsImmunomodulatoryAnti-inflammatoryNegative / threshold
Record detail Table 1: LR5 at 12.5 μg/mL, NO Inhibition Rate = 19.73 ± 0.39%.
- Measure
- percent_inhibition
- Value
- 19.73 ± 0.39%
- Target
- RAW264.7
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryNegative / threshold
Record detail Table 1: LR5 at 25 μg/mL, NO Inhibition Rate = 15.98 ± 0.22%.
- Measure
- percent_inhibition
- Value
- 15.98 ± 0.22%
- Target
- RAW264.7
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryNegative / threshold
Record detail Table 1: LR5 at 50 μg/mL, NO Inhibition Rate = 11.05 ± 0.08%.
- Measure
- percent_inhibition
- Value
- 11.05 ± 0.08%
- Target
- RAW264.7
- Assay
- Not reported by source
ImmunomodulatoryAnti-inflammatoryNegative / threshold
Record detail Table 1: LR5 at 100 μg/mL, NO Inhibition Rate = 8.79 ± 0.39%.
- Measure
- percent_inhibition
- Value
- 8.79 ± 0.39%
- Target
- RAW264.7
- Assay
- Not reported by source
Antioxidant 2 records
PMC10221837
2 observationsAntioxidantPositive observation
Record detail The IC50 values for the O2-· scavenging ability of the original and replacement rice peptides are shown in Figure 1A. Compared with the original pentapeptide LR5, the IC50 values of the replacement pentapeptides AR5 and LAR5 with reduced hydrophobicity increased from 2.6561 ± 0.0031 mmol/L to 6.985 ± 0.0002 mmol/L (p < 0.01) and 3.633 ± 0.0071 mmol/L (p < 0.01), respectively.
- Measure
- other
- Value
- 2.6561 ± 0.0031 mmol/L
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantPositive observation
Record detail Compared with the original pentapeptide LR5, the IC50 values of the replacement pentapeptides AR5 and LAR5 increased from 0.6476 ± 0.0032 mmol/L to 0.6702 ± 0.0019 mmol/L (p < 0.05) and 0.6561 ± 0.0025 mmol/L (p < 0.05), respectively.
- Measure
- other
- Value
- 0.6476 ± 0.0032 mmol/L
- Target
- Not reported by source
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001765, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00