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Primary-literature peptidehigh evidence

PEPDIA-PLP-000669

FCLYELAR

Aliases: FCLYELAR

Normalized sequence

FCLYELAR

8 amino acids

Release PepDiana-V1-20260620

27

Activity records

1

Source papers

20

Positive

7

Negative / threshold

Versioned scientific descriptors

Physicochemical properties and standard representations

calculated sequence and structure

Reported properties

No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.

Computed properties

physchem-20260620

Molecular weight

1014.198 Da

Molecular formula

C47H71N11O12S

Theoretical pI

5.9943

Net charge at pH 7

-0.248

GRAVY

0.675

Instability index

23.4

Aromaticity

0.25

Topological polar surface area

386.45 Å²

cLogP

-0.8471

Hydrogen-bond donors

15

Hydrogen-bond acceptors

13

Rotatable bonds

31

Method: PepDiana sequence/structure descriptor pipeline

Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6

Calculated: 2026-06-20T11:01:38.358971+00:00

Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200

Sequence representations

representations-20260620

ProForma 2.0

validated simple linear

FCLYELAR

HELM 2.0

validated simple linear

PEPTIDE1{F.C.L.Y.E.L.A.R}$$$$V2.0

Canonical SMILES

validated

CC(C)CC(NC(=O)C(CCC(=O)O)NC(=O)C(Cc1ccc(O)cc1)NC(=O)C(CC(C)C)NC(=O)C(CS)NC(=O)C(N)Cc1ccccc1)C(=O)NC(C)C(=O)NC(CCCNC(=N)N)C(=O)O

Isomeric SMILES

validated

CC(C)C[C@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@@H](N)Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O

Paper-level observations

Experimental evidence grouped by activity

Metabolic/Cardiovascular 27 records

PMC8412326

27 observations

Metabolic/CardiovascularACE InhibitorPositive observation

The most potent activity was exhibited by the synthesized peptide FCLYELAR, recording an IC50 value of 31.63 ± 0.15 μM.

Mention PMC8412326::PEP000001PMC8412326text

Measure: other
Value: 31.63 ± 0.15 μM
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

In the case of the FCLYELAR peptide, Ki measured a constant 0.0248 mM as shown in Fig 6.

Mention PMC8412326::PEP000001PMC8412326text

Measure: Ki
Value: 0.0248 mM
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

The F7 fraction provided the greatest ACE-inhibitory activity among those fractions tested, offering an IC50 value of 0.26 ± 0.02 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.26 ± 0.02 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Those fractions whose molecular weights did not exceed 0.65 kDa provided the best ACE-inhibitory activity (IC50 0.093 ± 0.004 μg/mL).

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.093 ± 0.004 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

For IC50 the experimental value was a little higher than the prediction at 7.14 ± 0.05 μg/mL.

Mention PMC8412326::PEP000001PMC8412326text

Measure: other
Value: 7.14 ± 0.05 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Run 19 provided the lowest experimental value for ACE-inhibitory activity at 6.87 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 6.87 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 3 also shows the ACE-inhibitory activity, with results in the range of 6.87 to 12.55 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 12.55 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 5: 0.65–3 kDa fraction IC50 0.303 ± 0.025 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.303 ± 0.025 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 5: 3–5 kDa fraction IC50 2.078 ± 0.424 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 2.078 ± 0.424 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 5: 5–10 kDa fraction IC50 42.640 ± 3.358 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 42.640 ± 3.358 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 5: >10 kDa fraction IC50 21.556 ± 0.891 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 21.556 ± 0.891 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 5: Unfractionated hydrolysate IC50 7.140 ± 0.051 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 7.140 ± 0.051 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F2 fraction IC50 > 0.28 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 0.28 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F3 fraction IC50 > 0.25 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 0.25 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F4 fraction IC50 > 0.29 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 0.29 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F5 fraction IC50 > 0.25 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 0.25 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F6 fraction IC50 > 0.16 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 0.16 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F8 fraction IC50 > 1.09 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: > 1.09 µg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F9 fraction IC50 1.60 ± 0.15 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 1.60 ± 0.15 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F10 fraction IC50 1.12 ± 0.05 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 1.12 ± 0.05 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F11 fraction IC50 0.34 ± 0.01 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.34 ± 0.01 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F12 fraction IC50 0.88 ± 0.01 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.88 ± 0.01 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F13 fraction IC50 0.97 ± 0.02 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 0.97 ± 0.02 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F14 fraction IC50 1.22 ± 0.11 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 1.22 ± 0.11 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F15 fraction IC50 1.70 ± 0.34 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 1.70 ± 0.34 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorPositive observation

Table 6: F16 fraction IC50 1.22 ± 0.20 μg/mL.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: 1.22 ± 0.20 μg/mL
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Metabolic/CardiovascularACE InhibitorNegative / threshold

Table 6: F1 fraction IC50 N.D.

Mention PMC8412326::PEP000001PMC8412326table

Measure: other
Value: N.D.
Target: Oryctolagus cuniculus
Assay: Not reported by source

Source publication

Citation and release provenance

Cite this identity as PepDiana record PEPDIA-PLP-000669, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.

Record updated: 2026-06-22T07:14:26.229247+00:00