AptaDB: Result

Interaction ID: 169

Aptamer sequence: TATAGACCACAGCCTGAGATTAACCACCAACCCAGGACT

Target unique ID: P04275

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_164	VWF R9.4	Protein	VWF_HUMAN	1.9 nM	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)	17854267

Aptamer Sequence:	TATAGACCACAGCCTGAGATTAACCACCAACCCAGGACT
The optimal secondary structure in dot-bracket notation:	..........((((((................)))).))
The centroid secondary structure in dot-bracket notation:	..........((((((................)))).))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_164	Description	VWF R9.4
Aptamer chemistry	DNA	Length	39 nt
GC content	48.7%	Molecular weight	12,122.63 Da
Molarity of 1 μg/μl solution	82.49 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TATAGACCACAGCCTGAGATTAACCACCAACCCAGGACT
Applications	Inhibit platelet aggregation in PFA-100 and ristocetin-induced platelet aggregation assays

Type	Detail	Type	Detail
Uniprot ID	P04275	Protein name	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Gene name(s)	VWF F8VWF	Organism	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Status	reviewed in uniprot database	Length	2813
Mass	309,265	Activity regulation	N/A
Involvement in disease	von Willebrand disease 1 (VWD1)	Catalytic activity	N/A
PDB ID(s)	1AO3;1ATZ;1AUQ;1FE8;1FNS;1IJB;1IJK;1M10;1OAK;1SQ0;1U0N;1UEX;2ADF;2MHP;2MHQ;3GXB;3HXO;3HXQ;3PPV;3PPW;3PPX;3PPY;3ZQK;4C29;4C2A;4C2B;4DMU;4NT5;5BV8;6FWN;6N29;7EOW;7F49;7KWO;7PMV;7PNF;
Function	Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V.

	Interaction ID	169
	Target type	Protein
	Target unique ID	P04275
	Activity	1.9 nM
	Binding Conditions/Buffer	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
	Assay	Binding affinities of VWF aptamers
	PubMed ID	17854267

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_222	DNA	AGCGACTGACGATCTTGAGTAAACCGCTCATCCACGTAGT	57.50%
Apta_593	DNA	CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC	56.82%
Apta_363	DNA	ATTGACCGCTGTGTGACGCAACACTCAAT	56.41%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	53.85%
Apta_968	DNA	TAACACGACAGACGTTCGGAGGTCGAACCCTGACAGCGTGGGC	53.49%
Apta_274	DNA	GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT	52.50%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	52.50%
Apta_448	DNA	TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC	51.79%
Apta_781	DNA	GTACGCCGCAAGACGAGTTGTGTATAAGCCGGC	51.28%
Apta_635	DNA	TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA	51.11%
Apta_638	DNA	TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCG	51.11%
Apta_641	DNA	TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG	51.11%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	51.02%
Apta_1162	DNA	AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT	51.02%
Apta_273	DNA	ATAAGCTGAGCATCTTAGATCCCCGTCAAGGGCAGCGTAA	50.00%
Apta_286	DNA	TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG	50.00%
Apta_355	DNA	GATGCAGCCACCTACATTGCAGGTTTTATAATACAACTACGCGCCGTTCGCAGGCTAGTT	50.00%
Apta_406	DNA	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT	50.00%
Apta_512	DNA	ATAGGACGATTATCGAAAATCACCAGATTGGACCCTGGTTAACGAT	50.00%
Apta_604	DNA	TCCTGACCTTAGCCTATGTTTTCTCTACCGGCTATGTTAC	50.00%

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