Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1077
Aptamer sequence: TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG
Target unique ID: P35270
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_992 | SP8 | Protein | SPRE_HUMAN | 47.0 nM | 50 mM Tris, pH 7.5, 150 mM NaCl and 2 mM MgCl2 | 32456943 |
Structure information of aptamer
| Aptamer Sequence: | TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG |
| The optimal secondary structure in dot-bracket notation: | ....(((..((..((......))))..))).....((((.((....)).))))......... |
| The centroid secondary structure in dot-bracket notation: | .............((......))............((((.((....)).))))......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_992 | Description | SP8 |
| Aptamer chemistry | DNA | Length | 62 nt |
| GC content | 54.8% | Molecular weight | 18,921.15 Da |
| Molarity of 1 μg/μl solution | 52.85 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG | ||
| Applications | Fabricate a competitive aptasensor for sepiapterin detection | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P35270 | Protein name | Sepiapterin reductase (SPR) (EC 1.1.1.153) |
| Gene name(s) | SPR | Organism | Sepiapterin reductase (SPR) (EC 1.1.1.153) |
| Status | reviewed in uniprot database | Length | 261 |
| Mass | 28,048 | Activity regulation | N/A |
| Involvement in disease | Dystonia, DOPA-responsive, due to sepiapterin reductase deficiency (DRDSPRD) | Catalytic activity | Reaction=L-erythro-7,8-dihydrobiopterin + NADP(+) = H(+) + NADPH + sepiapterin |
| PDB ID(s) | 1Z6Z;4HWK;4J7U;4J7X;4XWY;4Z3K;6I6C;6I6F;6I6P;6I6T;6I6V;6I79;6USN;7DSF; |
 |
Function | Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. |
Activity data
 |
Interaction ID | 1077 |
| Target type | Protein | |
| Target unique ID | P35270 | |
| Activity | 47.0 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 150 mM NaCl and 2 mM MgCl2 |
|
| Assay | The fluorescence binding affinity curves for six sepiapterin aptamers (a plot of the aptamer concentration versus the fluorescence intensity of the eluted aptamer solution). The dissociation constants were calculated via nonlinear regression analysis of the saturation curves and the error bars represent the standard deviation of the measurements |
|
| PubMed ID | 32456943 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_663 | DNA | CACGCACAGAAGACACCTACAGGGCCAGATCACAATCGGTTAGTGAACTCGTACGGCGCG | |
| Apta_1218 | DNA | CGACACCACACAGCAGTAAGCACTACAACATAGTGGTCTAGACTATCTCGGGCCGGTG | |
| Apta_981 | DNA | TATGGAGAGTCAATGAGTACAGTCTCGTCTCAATAACGGAGCCTCGTCCGTGCCCCCG | |
| Apta_340 | DNA | TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA | |
| Apta_175 | DNA | AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA | |
| Apta_728 | DNA | CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG | |
| Apta_1233 | DNA | TCACGAGAGAGCGAGAGCGCCCCCCCACCACAGCCGTCACCACCCTATTCCTCTGCCGTTG | |
| Apta_1266 | DNA | GCATCACACACCGATACTCACCCGCCTGATTAACATTAGCCCACCGCCCACCCCCGCTGC | |
| Apta_931 | DNA | ATACGACTCACTATTAGGGACCACATAAACATGCATCGCTCAATAACGGGATATTGTTGTGCTAGACTACTGACTACAA | |
| Apta_108 | DNA | AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA | |
| Apta_108 | DNA | AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA | |
| Apta_176 | DNA | AGCAGCACAGAGGTCAGATGTATGGTACATTCGAGCGAGCTAAACTAGGATTAAACTCACCCTATGCGTGCTACCGTGAA | |
| Apta_232 | DNA | AGCAGCACAGAGGTCAGATGCTATGAGCAGATCCGATAGAAAGTTAATGCCCTAGAACATCCTATGCGTGCTACCGTGAA | |
| Apta_698 | DNA | AGCAGCACAGAGGTCAGATGCACTCGGACCCCATTCTCCTTCCATCCCTCATCCGTCCACCCTATGCGTGCTACCGTGAA | |
| Apta_1112 | DNA | ATCCAGAGTGACGCAGCAGGGCACATTGTTCACACACAGATCACATTACGGAAAACACAACTACACGAAATGTCGTTGGTGGCCC | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_592 | DNA | CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC | |
| Apta_597 | DNA | TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG | |
| Apta_652 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGGCAGGGGGTGTGCGTG | |
| Apta_684 | DNA | GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG |
DEV TOOL
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