AptaDB: Result

Interaction ID: 168

Aptamer sequence: ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT

Target unique ID: P04275

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_166	VWF R9.3	Protein	VWF_HUMAN	1.2 nM	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)	17854267

Aptamer Sequence:	ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT
The optimal secondary structure in dot-bracket notation:	.....((......)).......(((((...))))).....
The centroid secondary structure in dot-bracket notation:	......................(((((...))))).....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_166	Description	VWF R9.3
Aptamer chemistry	DNA	Length	40 nt
GC content	50.0%	Molecular weight	12,375.76 Da
Molarity of 1 μg/μl solution	80.80 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT
Applications	Inhibit platelet aggregation in PFA-100 and ristocetin-induced platelet aggregation assays

Type	Detail	Type	Detail
Uniprot ID	P04275	Protein name	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Gene name(s)	VWF F8VWF	Organism	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Status	reviewed in uniprot database	Length	2813
Mass	309,265	Activity regulation	N/A
Involvement in disease	von Willebrand disease 1 (VWD1)	Catalytic activity	N/A
PDB ID(s)	1AO3;1ATZ;1AUQ;1FE8;1FNS;1IJB;1IJK;1M10;1OAK;1SQ0;1U0N;1UEX;2ADF;2MHP;2MHQ;3GXB;3HXO;3HXQ;3PPV;3PPW;3PPX;3PPY;3ZQK;4C29;4C2A;4C2B;4DMU;4NT5;5BV8;6FWN;6N29;7EOW;7F49;7KWO;7PMV;7PNF;
Function	Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V.

	Interaction ID	168
	Target type	Protein
	Target unique ID	P04275
	Activity	1.2 nM
	Binding Conditions/Buffer	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
	Assay	Binding affinities of VWF aptamers
	PubMed ID	17854267

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	57.50%
Apta_872	DNA	ACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCT	57.50%
Apta_187	DNA	ACCAACCCTTTACCTCTGAGATCTCCCAAT	55.00%
Apta_395	DNA	AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA	54.00%
Apta_421	DNA	ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA	53.06%
Apta_220	DNA	ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT	52.50%
Apta_240	DNA	TTTTTAATTAAAGCTCGCCATCAAATAGCTTT	52.50%
Apta_406	DNA	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT	52.50%
Apta_439	DNA	TAACACTCGATTCTCCTAGCCCGCTAGAAATTCCCCTCCC	52.50%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	52.17%
Apta_335	DNA	ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG	52.00%
Apta_636	DNA	TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA	51.11%
Apta_835	DNA	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC	51.11%
Apta_585	DNA	GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC	51.06%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	51.02%
Apta_989	DNA	ATGCGGGTACATCTCTCCAACCAGTCAAACTGCTATGATAAGACCTCATTCAACCCCGGTA	50.82%
Apta_181	DNA	CCGTGTAAGGTTTCAGTGGCTTATCCGAAGTAACCCGAGA	50.00%
Apta_185	DNA	ACCAAGGCTTTAGGTCTGAGATCTCCCAAT	50.00%
Apta_189	DNA	AGGAACCCTTTACCTCTGAGATCTCGGAAT	50.00%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	50.00%

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