AptaDB: Result

Interaction ID: 1140

Aptamer sequence: GCAGTTGATCCTTTGGATACCCTGG

Target unique ID: P15941

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1086	S2	Protein	MUC1_HUMAN	N	67 mM Glycine-KOH (pH 9.5 at 25 °C), 6.7 mM MgCl2, 10 mM 2-mercaptoethanol	25880833

Aptamer Sequence:	GCAGTTGATCCTTTGGATACCCTGG
The optimal secondary structure in dot-bracket notation:	.(((...((((...))))...))).
The centroid secondary structure in dot-bracket notation:	.(((...((((...))))...))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1086	Description	S2
Aptamer chemistry	DNA	Length	25 nt
GC content	52.0%	Molecular weight	7,663.94 Da
Molarity of 1 μg/μl solution	130.48 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GCAGTTGATCCTTTGGATACCCTGG
Applications	Detection of mucin 1 (MUC 1)

Type	Detail	Type	Detail
Uniprot ID	P15941	Protein name	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha); Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Gene name(s)	MUC1 PUM	Organism	Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Cancer antigen 15-3) (CA 15-3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha); Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)]
Status	reviewed in uniprot database	Length	1255
Mass	122,102	Activity regulation	N/A
Involvement in disease	MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence	Catalytic activity	N/A
PDB ID(s)	1SM3;2ACM;2FO4;5T6P;5T78;6FZQ;6FZR;6KX1;6TGG;7Q4I;
Function	The alpha subunit has cell adhesive properties.

	Interaction ID	1140
	Target type	Protein
	Target unique ID	P15941
	Activity	N
	Binding Conditions/Buffer	67 mM Glycine-KOH (pH 9.5 at 25 °C), 6.7 mM MgCl2, 10 mM 2-mercaptoethanol
	Assay	SWV responses of 1 mM S1 hybridized with different concentration of S2
	PubMed ID	25880833

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_673	DNA	GCAGTCCTAGATCGGGTGTGGCTGGCTTGG	63.33%
Apta_700	DNA	GCAGGGGCGTTCGGGGGGTACCGCTGC	59.26%
Apta_542	DNA	GCACGTGTTGTCTCTCTGTGTCTCGTGC	57.14%
Apta_671	DNA	GCATCTGATCGGGTGTGGGTGGCGTAAAGG	56.67%
Apta_676	DNA	TCAGTCCCGATCAGGTGTGGGTGGCATTGG	56.67%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	56.67%
Apta_544	DNA	GCGGGCGGTTGTATAGCGG	56.00%
Apta_701	DNA	GTACTGGCGTTCGGGGGGTACCGCTGC	55.56%
Apta_543	DNA	GGCACGTGTTGTCTCTCTGTGTCTCGTGCC	53.33%
Apta_682	DNA	CCTTGTAGATCGGGTGTGGTTTGGCGTAGG	53.33%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	53.33%
Apta_1083	DNA	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT	53.33%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	52.94%
Apta_506	DNA	AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG	52.63%
Apta_211	DNA	GGTTGGTGTGGTTGG	52.00%
Apta_538	DNA	GTGTTGTCTCTCTGTGTCTC	52.00%
Apta_539	DNA	CGTGTTGTCTCTCTGTGTCTCG	52.00%
Apta_540	DNA	ACGTGTTGTCTCTCTGTGTCTCGT	52.00%
Apta_672	DNA	GGGGTGAAACGGGTCCCG	52.00%
Apta_757	DNA	GGTGGTGGTGGTTGTGGTGGTGG	52.00%

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